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KMID : 0613820110210091219
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Journal of Life Science
2011 Volume.21 No. 9 p.1219 ~ p.1225
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Global Gene Expression Changes by Several Phytochemicals in Human Colorectal Cancer Cell
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Park Min-Hee
Kwak Eun-Hee
Sohn Ho-Yong
Eling Thomas
Kim Jong-Sik
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Abstract
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Phytochemicals, non-nutrient chemicals derived from plants, have been shown to have anti-inflammation, anti-oxidation, and chemopreventive effects. In the current study, we investigated whether five different phytochemicals (resveratrol, genistein, epicatechin gallate, diallyl disulfide, and caffeic acid phenethyl ester) alter cell growth and gene expression in human colorectal cancer HCT116 cells. Using a cell viability assay, we found that each of the phytochemicals tested inhibited HCT116 cell growth in a dose-dependent manner. Additionally, using human oligo DNA microarray analysis, we found that only six genes were commonly up-regulated and seven genes were commonly down-regulated in response to each phytochemical treatment. For the commonly up-regulated genes, the microarray analysis was confirmed by reverse transcription.PCR using gene-specific primers. In addition, NAG-1 protein was up-regulated by all treated phytochemcials. The results of this study may help to enhance our understanding of the general molecular mechanisms of chemoprevention that are mediated by phytochemicals in human colorectal cancer.
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KEYWORD
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Phytochemical, chemoprevention, human colorectal cancer, oligo DNA microarray, gene expression
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